Information about a laboratory test
A negative HBsAg result means surface antigen was not detected, but timing and other HBV markers matter.
HBsAg checks whether hepatitis B surface antigen is detectable in blood.
HBsAg is used with anti-HBs and total anti-HBc to evaluate hepatitis B infection status.
A negative HBsAg result means surface antigen was not detected, but timing and other HBV markers matter.
A positive/reactive HBsAg result can be consistent with current HBV infection and should be interpreted with the rest of the hepatitis B panel.
Start with the performing laboratory's reference or decision range, assay method, specimen and reporting unit. A single result usually does not establish a diagnosis on its own.
Sourced reference/decision context for HBsAg
This is sourced reference or clinical-context information; the user's own laboratory report, assay method and clinical context take priority.
Reference ranges may vary by laboratory, method used, age, sex, pregnancy status, and clinical context. The reference range on your own laboratory report takes precedence.
HBsAg alone does not fully define immunity or past infection; CDC screening uses HBsAg, anti-HBs and total anti-HBc together.
Clinical Testing and Diagnosis for Hepatitis B — Centers for Disease Control and Prevention
U.S. government clinical guidance · 2025 · HBsAg, anti-HBs, total anti-HBc triple-panel interpretation · Verified: Aug 12, 2026
Testing for Hepatitis C — Centers for Disease Control and Prevention
U.S. government clinical reference · 2025 · HCV antibody screening and follow-up HCV RNA testing · Verified: Aug 12, 2026
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